| Course: Biotechnology 2 | |||
| 1st 9 Weeks | 2nd 9 Weeks | 3rd 9 Weeks | 4th 9 Weeks |
Unit(s): 1, 2, 3 Identifying and Optimizing a Biotech Product Unit(s): 4, 5 Spectrophotometer for DNA and Protein Analysis | Unit(s): 6, 7 Recombinant DNA Production | Unit(s): 8, 9, 10 Biotech Product Purification and Bringing to Market | Unit(s): 11, 12 Agricultural Biotechnology
Unit(s): Biomedical Biotechnology |
Unit 1: Intro to laboratory safety • Flinn Lab Safety
Unit 2: Identifying a Potential Biotech Product Biotechnology Science for the New Millennium Textbook Chapter 6 • Sources of potential products • Use of assays • ELISA • Western blot • Products in nature • Recombinant DNA Unit 3: Assay Development Biotechnology Science for the New Millennium Lab Manual Chapter 6 • Searching for amylase-positive bacteria • Assaying for starch and sugar • Assaying for amylase activity Unit 4: Spectrophotometer and other Analytical Tools Biotechnology Science for the New Millennium Textbook Chapter 7 • Spec to detect molecules • Spec to measure protein • Spec to measure DNA • Other “Spec-like” tools • Applications in Biotech
Unit 5: Using the Spec to Measure DNA and Protein Biotechnology Science for the New Millennium Lab Manual Chapter 7 • Intro to Spec • Spec practice • Analyzing amylase • Determining amylase concentration • Determining milk pI | Unit 6: Production of a Recombinant Biotech Product Biotechnology Science for the New Millennium Textbook Chapter 8 • Overview of Genetic Engineering • Using rDNA for Transformation • Transforming Cells • Cloning for Manufacturing Purposes • Fermentation, Manufacturing, and GMP • Retrieving Plasmids after Transformation Unit 7: Recombinant Protein Production Biotechnology Science for the New Millennium Lab Manual Chapter 8 • Restriction analysis of Lambda Phage DNA • Transformation of E. Coli w GFP (Carolina kit) • Growing and Monitoring bacterial Cultures • Scaling-up production
Fall CCA | Unit 8: Bringing a Biotech Product to Market Biotechnology Science for the New Millennium Textbook Chapter 9 • Intro to biomanufacturing • Using chromatography to study molecules • Column Chromatography • Product Quality Control • Marketing and Sales Unit 9: Protein Product Purification Biotechnology Science for the New Millennium Lab Manual Chapter 9 • Harvesting amylase from bacterial cultures • Dialysis of proteins in different buffers Unit 10 Mock Interviews • Resume review • College/graduate school • Entry-level job | Unit 11: Introduction to Plant Biotechnology Biotechnology Science for the New Millennium Textbook Chapter 10 • Intro to plant propagation • Basic plant anatomy • Plant growth, structure, and function • Intro to plant breeding • Asexual plant propagation Unit 12: Plant Biotechnology Biotechnology Science for the New Millennium Lab Manual Chapter 10
• Sweet potato propagation • Flower dissection • Seed dissection • Seed germination • Wisconsin Fast Plants as a model • Asexual plant propagation Unit 13: Medical Biotechnologies Biotechnology Science for the New Millennium Textbook Chapter 12 • Drug Discovery • Creating Pharmaceuticals through chemistry • Creating Pharmaceuticals through Peptide and DNA Synthesis • Recent Advances in Medical Biotechnology Unit 14: Obtaining Molecules of Pharmaceutical Interest Biotechnology Science for the New Millennium Lab Manual Chapter 12 • Testing plant substances as potential medicines • Using a spec to determine caffeine concentration • Melting point determinations as a quality control test • Extraction of Salicin from willow Spring CCA |
Required Project: 6c Assaying for Starch and Sugar (p. 126) Required Project: Determining the Concentration of Amylase in Solution (p. 153) | Required Project: Transformation of E. Coli with GFP (Carolina kit) | Required Project: Mock Interviews | Required Project: Sweet Potato Propagation (p. 208) Required Project: Using a Spec to determine Caffeine Concentration (p. 277) |
| Employability Skills: | |||
Cycle 1: -Professionalism -Teamwork -Problem Solving 127.15 (2)(A) 127.15 (2)(B) 127.15 (2)(C) 127.15 (2)(D) 127.15 (2)(E) 127.15 (2)(F) 127.15 (2)(G) 127.15 (2)(H) 127.15 (2)(I) 127.15 (2)(J) 127.15 (2)(L) | Cycle 2: - Job Seeking Skills 127.15 (2)(A) 127.15 (2)(H) 127.15 (2)(I) 127.15 (2)(J) 127.15 (2)(K) 127.15 (2)(L) | Cycle 3: -Communication Skills -Self Management Skill 127.15 (2)(A) 127.15 (2)(B) 127.15 (2)(C) 127.15 (2)(D) 127.15 (2)(E) 127.15 (2)(F) 127.15 (2)(G) 127.15 (2)(H) 127.15 (2)(I) 127.15 (2)(J) 127.15 (2)(L) | Cycle 4: -Civic and Social Responsibility 127.15 (2)(F) 127.15 (2)(G) 127.15 (2)(H) 127.15 (2)(I) 127.15 (2)(L) |
| CHOICES 360 Activities L3 | |||
| Basic Skills Survey OR | Job Interview Practice OR | ||
| My Goals and Action Plans | Saved programs and majors |